Intracellular recording: safety with glass and other sharps
Your safety is the most important. Don't hesitate to ask if you are not sure how to do some part of your project safely. Either find me or (if not quite so urgent) e-mail me
Break the 1mm diameter thin-wall electrode glass firmly between strong fingernails; use a glass cutter for larger diameter and thicker glass.
Pull the electrodes carefully; don't blow away the little bits of glass - brush them up!
During filling and use, Keep the points of electrode and syringe needle away from you
After use place the electrodes in a Sharps bin
Dispose of all broken glass in a marked glass/sharps container
Don't wipe bits of broken glass off the desk or equipment with your hands. Use a brush!
Pins, scalpels and forceps
Watch for pins that fly away, don't lose them or put your fingers/ arms/ feet /legs on them. Put bent pins carefully in the sharps bin
Snail dissection rarely requires a scalpel, so use scissors and forceps instead. Put the tips of any scalpels you use in a plastic pipette tip when you're not using them.
Try not to drop forceps; their points can hurt you!
Instructions for dissection and impalement | Safety in Chris lab: Overview | Biology Department safety pages
Page last revised by Chris Elliott on September 3, 1997. Mail any comments to firstname.lastname@example.org