SSC Autumn Term 2010
Week 1) Introduction to nervous system of invertebrates, nerve conduction and synapses. Introduction to apparatus. Lab courtesy and safety
Week 2) Cockroach nerve cord, nerve conduction, use of natural and electrical stimuli, oscilloscope as recorder
Data Responses to: Sub-threshold stimulus Supra-threshold stimulus (scale 1 ms/square horizontally, stimulus trace, 0.5 V/square vertically)
Week 3) Cockroach leg: sensory transduction – what information is encoded?
Protocol Data (in zipfile) and excellent trace, showing transient big spikes from spine and little ones which persist from the chordotonal organs.
Week 4) Locust breathing: motor control
Protocol Data (in Zipfile)
Weeks 5&6) Drosophila eye photoreceptors: computer based stimuli-acquisition
Protocol Data: Response to blue light
Week 7) CNS: Intracellular recording technique from snail neurons
Protocol Data: 10_11_11h39m.flz 10_11_11h52m.flz 10_11_11h43m.flz 10_11_11h1m.flz You will need the latest version of Dasyview (> 2.1.4) to read these files
Week 8) Fly neuromuscular junction – confocal microscopy
Protocol Data (You will need to unzip the file and then use the Zeiss software or ImageJ to read the files )
Week 9) PowerPoint Presentation of one experiment
Week 10) Report on write-up and feedback
Suggestions for PPT and writeup (2 pages max text, + figures each on a separate page with a short legend):
Introduction: Background to organism/part of nervous system used.
Method: very brief, outline in 1 or 2 sentences (we followed the protocol...)
Results: show data recordings, describe the prominent features, e.g. firing rate of action potentials, effect of maintained v transient stimuli
Discussion: what general conclusions do you draw? Any similar data form other experiments in this series/literature?
Chris Elliott 17 Nov 2010