|Proboscis extension response|
|We have been filming flies with defective Proboscis extension responses (PER) - sample videos are here|
|Fly being presented with droplet of sucrose, proboscis retracted, legs not yet in the droplet||Same fly, now with the legs having touched the sucrose droplet, and having consequently extended the proboscis to drink from the sucrose solution|
|Watch the movie here !!|
|Measuring the output of a single twitch
muscle in vivo
Most muscle work measures muscles isolated from the organism, but in Drosophila we can measure the output of the jump muscle in the restrained fly - no dissection needed. Here's a sketch:
and here is the sample output:
We have used this to measure the reduction in force in mutants with defective muscle proteins; details here (open access) and with defective neurotransmission (Invertebrate Neuroscience online - Springer abstract and link to full text; author pdf manuscript, 750Kbytes). This paper (Methods, in press, author manuscript) gives details of th method, and has a video showing how the setup works.
- flies that don't synthesise octopamine jump less well!
Details are available on:
|Reproductive Tracts of the fly|
flies need octopamine (but not tyramine) to lay eggs
|Males need serotonin for ejaculation?|